flag peptide molecular weight 1013.0 g/mol - where-can-i-get-tesamorelin-peptide Molecular Weight Unveiling the Molecular Weight of the FLAG Peptide: A Comprehensive Guide

fix-filler-peptide The FLAG peptide, a widely recognized epitope tag, plays a crucial role in various biological research applications, particularly in protein purification and detection3X FLAG Peptide. Understanding its molecular weight is fundamental for accurate experimental design and data interpretation. This article delves into the precise molecular weight of the FLAG peptide, its variations, and related considerations, drawing upon established scientific data and common research practicesFLAG peptide (5 mg).

At its core, the FLAG peptide is a short amino acid sequence, most commonly represented as DYKDDDDK.FLAG Tag Protein Purification | Sino Biological This sequence is a hallmark of the FLAG epitope, a trademarked tag widely utilized for monitoring protein levels and facilitating their isolationDYKDDDDK Peptide ; Molecular Formula, C41H60N10O ; Molecular Weight,1012.97; Purity, > 95% ; Solubility, Soluble in Ultrapure water under 1mg/ml ; Form .... When referring to the FLAG peptide molecular weight, the most frequently cited figure is approximately 1012FLAG peptide (5 mg).97 Da (Daltons).DYKDDDDK Peptide This value is consistently reported across numerous scientific databases and product specifications, underscoring its reliability. Variations in this figure, such as 1013.0 g/mol, 1012.96 Da, and 1012.99, are essentially equivalent due to rounding or minor computational differences, all pointing to the same fundamental molecular mass.

The molecular formula associated with this standard FLAG peptide is C41H60N10O20Flag peptide. This formula details the elemental composition, providing a foundation for calculating the precise molecular weight. The peptide itself is typically synthesized and often supplied as a lyophilized white powder, a common format that ensures stability during storage and transport.Product Quantity. 5 mg, 100 mg, 1 gram ; Purity. >98% ; Catalog Number. LT12022 ; Molecular Weight.1013; Formula. DYKDDDDK. For short-term storage, refrigeration at 2-8°C for up to six months is recommended, while long-term storage at -20°C is standard practice to maintain its integrityThepeptidesequence of theFLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). Additionally,FLAG-tags may be used in tandem, commonly the ....

Beyond the single FLAG peptide, researchers also frequently encounter and utilize multi-copy versions, such as the 3x FLAG peptide. This variation, as the name suggests, consists of three tandem repeats of the FLAG epitope. Consequently, its molecular weight is significantly higher. The 3x FLAG peptide molecular weight is commonly cited around 28613x FLAG peptide (5 mg).87 Da or 2861.9 daltonsThe Triple FLAG® Peptide is a synthetic peptide of 23 amino acid residues (molecular weight of 2861.9 daltons). The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated .... The molecular formula for this larger construct is C120H169N31O49S. Similar to its single counterpart, the 3x FLAG peptide is typically supplied as a white powder and requires cold storage. It is important to note that some sources may list slightly different molecular weights for the 3x version, such as 2,861.87 Da, 3002作者：NK Reid·2025—The DYKDDDDK-peptideepitope, trademarked as theFLAGepitope, is a commonly used epitope tag. It is often used for monitoring protein levels ....88, or even 2975.84, which can arise from variations in the exact sequence or counter-ions.

The application of FLAG tags is not limited to peptides.Product Quantity. 5 mg, 100 mg, 1 gram ; Purity. >98% ; Catalog Number. LT12022 ; Molecular Weight.1013; Formula. DYKDDDDK. In some instances, recombinant proteins may be expressed with an N-terminal FLAG tag, and in such cases, the total molecular weight of the tagged protein will be the sum of the protein's intrinsic molecular weight and the tag's contribution. For example, a recombinant protein might be described as having a molecular weight of 111.1) was expressed in Sf9 cells and contains an N-terminal 6×His and Flag tag with a molecular weight of111.3 kDa. The recombinant protein is >30% pure by ...3 kDa due to the inclusion of a 6xHis and FLAG tag.

The utility of the FLAG peptide extends to competitive elution strategies during protein purification. Researchers often employ competitive elution with Flag peptides, using either a 1x FLAG peptide or a 3x FLAG peptide to dislodge target proteins that have bound to an affinity matrix. This technique leverages the specific binding interaction between the FLAG epitope and its corresponding antibody or resin.

In summary, the FLAG peptide molecular weight is a critical piece of information for researchers. While the standard FLAG peptide hovers around 1012.97 Da, the 3x FLAG peptide is approximately 2861.87 Da. Understanding these values, along with the associated molecular formula and recommended storage conditions, ensures the successful implementation of FLAG tag technology in diverse molecular biology experiments, from protein expression and detection to purification and analysis. The commonality of the FLAG tag and its associated peptide in scientific literature and commercial offerings highlights its enduring importance in the field.